SINGLE-CELL SEQUENCING ANALYSIS AND MULTIPLE MACHINE-LEARNING MODELS REVEALED THE CELLULAR CROSSTALK OF DENDRITIC CELLS AND IDENTIFIED FABP5 AND KLRB1 AS NOVEL BIOMARKERS FOR PSORIASIS

Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis

Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis

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BackgroundPsoriasis is an immune-mediated disorder influenced by environmental factors on a genetic basis.Despite advancements, challenges persist, including the diminishing efficacy of biologics and small-molecule targeted agents, alongside managing recurrence and psoriasis-related comorbidities.Unraveling the underlying pathogenesis and identifying valuable biomarkers remain pivotal for diagnosing and treating psoriasis.MethodsWe employed a series of bioinformatics (including single-cell sequencing data analysis and machine learning techniques) and statistical methods to integrate and analyze Bracelet multi-level data.We observed the cellular changes in psoriatic skin tissues, screened the key genes Fatty acid binding protein 5 (FABP5) and The killer cell lectin-like receptor B1 (KLRB1), evaluated the efficacy of six widely prescribed drugs on psoriasis treatment in modulating the dendritic cell-associated pathway, and assessed their overall efficacy.

Finally, RT-qPCR, immunohistochemistry, and immunofluorescence assays were used to validate.ResultsThe regulatory influence of dendritic cells (DCs) on T cells through the Sports Bra CD70/CD27 signaling pathway may emerge as a significant facet of the inflammatory response in psoriasis.Notably, FABP5 and KLRB1 exhibited up-regulation and co-localization in psoriatic skin tissues and M5-induced HaCaT cells, serving as potential biomarkers influencing psoriasis development.ConclusionOur study analyzed the impact of DC-T cell crosstalk in psoriasis, elucidated the characterization of two biomarkers, FABP5 and KLRB1, in psoriasis, and highlighted the promise and value of tofacitinib in psoriasis therapy targeting DCs.

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